Immunogold identification of prolactin cells of goats in anoestrus, pregnancy and milk production: ultrastructural variations
1992
Sanchez, J. | Bernabe, A. | Navarro, J.A. | Gomez, M.A. | Gómez, J.
Prolactin (PRL) cells of the goat adenohypophysis have been identified by the IgG-gold procedure with anti-sheep PRL serum. The secretion of these cells show; differences in size and labelling in the three reproductive stages under study. Cells containing PRL can be grouped into low secretory activity cells (PRL-I) and high secretory activity cells (PRL-II) regarding their ultrastructure and functional significance PRL were the most frequent cells n animals at the anoestrus stage, presenting numerous secretory granules and scarce development of the rough endoplasmic reticulum (RER) and Golgi complex (GC). At anoestrus and pregnancy stages there are frequent granule fusions, and the hormonal content partially disappears. perhaps by digestion. PRL-II cells were the most numerous at the lactating stage. presenting a moderate number of secretory granules and well-developed GC and RER. Some PRL-II cells of lactating animals exhibited scarce granules and numerous exocytosis suggesting a high secretory activity. In both anoestrus and pregnancy stages most granules range in diameter from 450 to 750 nm. in contrast to the lactating stage in which most granules range in diameter from 150 to 450 nm.
显示更多 [+] 显示较少 [-]