Oceanobacter mangrovi Sp. Nov., a Novel Poly-β-hydroxybutyrate Accumulating Bacterium Isolated from Mangrove Sediment
2022
Huang, Zhaobin | Huang, Yuanyuan | Lai, Qiliang | Chen, Xinlan | Dong, Chunming | Huang, Xiaozhou
A Gram-stain-negative, rod-shaped, motile, mesophilic, and aerobic bacterial strain, designated SM2-42 ᵀ was isolated from a mangrove sediment. Catalase activity and oxidase activity were positive. Growth was observed at 20 °C–40 °C, pH 6.0–8.0, and in the presence of 0.5–5.0% NaCl. Cells of strain SM2-42 ᵀ contained poly-β-hydroxybutyrate granules. The 16S rRNA gene of strain SM2-42 ᵀ had maximum sequence similarity with Oceanobacter kriegii 197 ᵀ of 97.1%. Phylogenetic analysis based on 16S rRNA gene sequence and 120 conserved concatenated proteins indicated that strain SM2-42 ᵀ was affiliated to the genus Oceanobacter and formed a monophyletic branch with O. kriegii 197 ᵀ. The average nucleotide identity and digital DNA-DNA hybridization values between strain SM2-42 ᵀ and O. kriegii 197 ᵀ were 76.43% and 21.60%, respectively. The major isoprenoid quinone was Q-8. The major fatty acids (> 10%) comprised C₁₆:₀, summed feature 8 (C₁₈:₁ω7c and C₁₈:₁ ω6c), C₁₈:₀, and summed feature 3 (C₁₆:₁ω7c and/or C₁₆:₁ ω6c). The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and two unidentified lipids. The draft genome size was 5,115,008 bp with DNA G + C content of 54.3%. Based on phylogenetic analyses and whole genomic comparisons, strain SM2-42 ᵀ represented a novel species, for which the name Oceanobacter mangrovi sp. nov. was proposed. The type strain was SM2-42 ᵀ (= MCCC 1K06300ᵀ = KCTC 82938 ᵀ).
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