Quantification of xylanase inhibitors by immunodetection: the way forward
2006
Furniss, C. | Goldson, A. | Aliwan, F. | Chanliaud, E. | Juge, N.
Wheat varieties exhibit a high level of variation in their xylanase inhibitor content with inter-individual variations relative to the type of inhibitor. These variations may have a significant impact on the functionality of exogenous xylanases in cereal processing. So far, attempts to measure accurate levels of xylanase inhibitors in wheat varieties have been restricted by the detection method based on activity assays. This paper reports the use of antibodies as an alternative method to measure xylanase inhibitor levels in environmentally controlled wheat varieties. This method could be extended to other type of xylanase inhibitors in order to aid in selecting the wheat variety or the enzyme used in the application with regard to its sensitivity to the different inhibitors.
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