Characterization of a NADH-Dependent Glutamate Dehydrogenase Mutant of Arabidopsis Demonstrates the Key Role of this Enzyme in Root Carbon and Nitrogen Metabolism
2012
Fontaine, Jean-Xavier | Tercé-Laforgue, Thérèse | Armengaud, Patrick | Clément, Gilles | Renou, Jean-Pierre | Pelletier, Sandra | Catterou, Manuella | Azzopardi, Marianne | Gibon, Yves | Lea, Peter J. | Hirel, Bertrand | Dubois, Frédéric
A third isoenzyme of Glu dehydrogenase (GDH) is expressed in mitochondria of Arabidopsis root companion cells. A GDH triple mutant differed greatly from the wild type in continuous darkness, suggesting that the main function of the enzyme is to provide 2-oxoglutarate for the tricarboxylic acid cycle, leading to an accumulation of Ala, γ-aminobutyrate, and Asp in both roots and leaves.
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