High-performance liquid chromatography of oleanane-type triterpenes
1984
Burnouf-Radosevich, M. | Delfel, N.E.
Seven oleanane-type triterpenes and sitosterol, which may be present together in natural mixtures, were successfully resolved by normal phase high-performance liquid chromatography on a silica gel column. A rapid isocratic separation was achieved using a ternary solvent system of hexane-isopropanol methanol (96:3.5:0.5). Derivatization was not required for compounds that were detected by UV absorption at 210 nm. This method, applied to qualitative and quantitative analysis of triterpenes extracted from seeds and callus tissue culture of Chenopodium quinoa, has proved to be efficient, highly reproducible and sensitive.
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