Facile and sensitive chemiluminescence detection of H₂O₂ and glucose by a gravity/capillary flow and cloth-based low-cost platform
2017
Li, Huijie | Wang, Dan | Liu, Cuiling | Liu, Rui | Zhang, Chunsun
This paper describes the development of a cloth-based CL strategy that can quantify H₂O₂ or glucose in a low-cost, easy-to-use, rapid and sensitive manner. Wax screen-printing is applied to fabricate the ultralow-cost cloth-based devices, and thus the as-prepared sensors have a very low cost (about $0.01 per assay). Moreover, an inexpensive CCD camera is used to image the light from the gravity/capillary flow-triggered enzyme-catalysed oxidation of luminol by H₂O₂. Under optimized conditions, the developed CL platform can fulfill the determination of H₂O₂, with a linear range of 0.01–10 mM and a detection limit of 9.07 μM. Similarly, glucose can be detected in the range of 0.01–10 mM, with a detection limit of 9.74 μM. Additionally, the RSDs of these two assays are 3.36%, 3.66% and 2.64% for 0.01, 0.1 and 5 mM H₂O₂, and 3.01%, 2.80% and 3.68% for 0.1, 1 and 5 mM glucose, respectively. Moreover, the devices can maintain ∼95% of their initial response for three weeks, and tolerate a series of interfering species at reasonable concentrations. Therefore, the proposed platform has an acceptable sensitivity, linear range, reproducibility and stability. Finally, this platform is successfully applied to the detection of H₂O₂ in milk and glucose in human serum.
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