Influence of growth temperature on fatty acids and phospholipids of Steinernema riobravis infective juveniles
1997
Abu Hatab, M.A. | Gaugler, R.
1. The entomopathogenic nematode Steinernema riobravis grows and reproduces over a wide temperature range (15, 20, 25, and 30 degrees C) ID the insect host Galleria mellonella. 2. The lipid content of S. riobravis varied in amount and composition. PE and PC were the two major constitutive classes of polar lipids whereas triglycerols were the major constitutive classes of neutral lipids. Lipid content of nematodes grown at 15 degrees C was marginally lower than at other growth temperatures. Nematodes accumulated higher proportions of saturated fatty acids when grown at high temperature (30 degrees C). 3. The ability of S. riobravis to adjust its lipid and fatty acid composition in response to growth temperature could contribute to thermal tolerance. We recommend culturing S. riobravis at 25-30 degrees C. At these temperatures more lipids are accumulated which may improve the physiological quality of S. riobravis compared to nematodes produced at lower temperatures (15-20 degrees C). In addition the growth and development cycle at high growth temperature in the insect cadaver is shorter compared to low temperature, which reduces the cost of mass production if applied in fermentation.
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