Micropropagation of the medicinal plant, Scilla natalensis Planch
1998
McCartan, S.A. | Van Staden, J.
Primary bulb explants of Scilla natalensis were cultured in vitro on modified MS medium. Some of these explants initiated shoots, which provided a sterile source of secondary leaf and bulb explants. The secondary explants responded similarly to various combinations of plant growth regulators. Shoots were initiated spontaneously on medium containing no plant growth regulators. The number of shoots initiated was increased by the addition of kinetin or thidiazuron (TDZ) alone, but was reduced by the addition of indole-3-acetic acid (IAA) or naphthalene acetic acid (NAA) alone. Optimal shoot initiation occurred on medium containing 1 to 2 mg l-1 kinetin and 1 to 2 mg l-1 IAA. These shoots were rooted on medium containing 1 mg l-1 IAA. The plantlets were successfully acclimatised in the misthouse/shadehouse.
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