Reduction with tris(2-carboxyethyl)phosphine (TCEP) enables the use of an S-sulphonate protecting group for thiol-mediated bioconjugation

Maret, Barbara; Regnier, Thomas; Rossi, Jean-Christophe; Garrelly, Laurent; Vial, Laurent; Robert, Pascal

Reduction with tris(2-carboxyethyl)phosphine (TCEP) enables the use of an S-sulphonate protecting group for thiol-mediated bioconjugation

2014

Herein, we demonstrate the effectiveness of the water-friendly S-sulphonate group as an alternative to traditional thiol protecting groups for subsequent deprotection–bioconjugation reactions, under conditions that are compatible with the use of biochemical samples.

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