Molecular Identification and Genetic Diversity of Fusarium species Associated with Onion Fields in Turkey
2011
Bayraktar, Harun | Dolar, Fatma Sara
To identify Fusarium species associated with diseases of root and basal plate of onion, surveys were conducted in seven provinces of Turkey in 2007. Samplings were performed in 223 fields, and 332 isolates belonging to 7 Fusarium spp. were obtained. The isolates were identified as F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. proliferatum, F. redolens, and F. culmorum based on morphological and cultural characteristics. Also, species-specific primers were used to confirm the identity of Fusarium species. F. oxysporum was the most commonly isolated species, comprising 66.57% of the total Fusarium species. F. redolens was identified for the first time in onion-growing areas of Turkey. Selected isolates of each species were evaluated for their aggressiveness on onion plant. F. oxysporum, F. solani, F. acuminatum, F. proliferatum, and F. redolens were highly pathogenic, causing severe damping-off on onion plants cv. Texas Early Grano. Inter-simple sequence repeats (ISSR) markers revealed a high degree of intra- and interspecific polymorphisms among Fusarium spp.
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