Pseudomonas pharmafabricae sp. nov., Isolated From Pharmaceutical Wastewater
2018
Yu, Xiao-Yun | Zhai, Jing-Yu | Wu, Chen | Zhang, Chong-Ya | Shi, Jie-Ying | Ding, Lin-Xian | Wu, Min
A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated ZYSR67-Zᵀ, was isolated from a pharmaceutical wastewater sample collected from a chemical factory in Zhejiang, China. The strain was motile by a single polar flagellum and grew at 4–42 °C (optimum, 35 °C), pH 5.0–9.0 (optimum, 6.0) and 0–5.0% (w/v) NaCl (optimum, 2.0%). Based on multilocus sequence analysis using 16S rRNA, gyrB, rpoB and rpoD, the strain ZYSR67-Zᵀ formed a distinct phylogenetic group in the genus Pseudomonas. The average nucleotide identity values between strain ZYSR67-Zᵀ and the closely related 10 type strains of the Pseudomonas species were 75.8–78.6%. The in silico DNA–DNA hybridization values indicated that strain ZYSR67-Zᵀ and the type strains of the Pseudomonas shared 21.4–23.1% DNA relatedness. The predominant isoprenoid quinone system was ubiquinone-9 while ubiquinone-8 was present in trace amounts. The major fatty acids (> 10%) identified were C₁₂:₀, C₁₆:₀, C₁₈:₁ ω7c and summed features 3 (C₁₆:₁ ω7c and/or iso-C₁₅:₀ 2OH). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 62.6 mol%. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain ZYSR67-Zᵀ was proposed to represent a novel species of the genus Pseudomonas, named Pseudomonas pharmafabricae sp. nov.. The type strain is ZYSR67-Zᵀ (= CGMCC 1.15498ᵀ = JCM 31306ᵀ).
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