Stability analysis of the Lactococcus lactis DRC1 lactose plasmid using pulsed-field gel electrophoresis
1993
Ward, A.C. | Hillier, A.J. | Davidson, B.E. | Powell, I.B.
Pulsed-field agarose gel electrophoresis of SmaI digests of genomic DNA was used to examine lactose plasmid copy number and stability in Lactococcus lactis. In L. lactis strain DRC1, the plasmid was found to exist as a single-copy plasmid. Transconjugants of strain HID113 carrying this plasmid were unstable. Variants were isolated with improved phenotypic stability resulting from improved maintenance of the lactose plasmid or from integration of part of the plasmid into the lactococcal chromosome.
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书目信息
出版者
Springer Netherlands
其它主题
Pulsed field electrophoresis; Gene amplification; Conjugation; Genetic; Bacterial; Phenotype; Isolation & purification; Phenotype; Pulsed-field; Lactococcus lactis subsp. lactis bv. diacetylactis
语言
英语
类型
Journal Article; Text
2024-02-27
MODS