Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots

Johnsen, K.; Enger, O.; Jacobsen, C.S.; Thirup, L.; Torsvik, V.

Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots

1999

Johnsen, K. | Enger, O. | Jacobsen, C.S. | Thirup, L. | Torsvik, V.

We used a quantitative PCR method targeting 16S ribosomal DNA using competitive PCR for specific detection of indigenous Pseudomonas DNA in soil hot spots. The amount of Pseudomonas DNA corresponded to the number of culturable Pseudomonas bacteria on Gould's S1 agar. This represents the first use of PCR for quantification of indigenous bacteria in more than one sample of soil.

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AGROVOC关键词

agar analysis bacteria culture media dna genetics methods polymerase chain reaction population dynamics pseudomonas quantitative polymerase chain reaction ribosomal dna rna soil

书目信息

发表于

Applied and environmental microbiology

卷 65 期 4 页码 1786 - 1789 ISSN 0099-2240

出版者

Multidisciplinary Digital Publishing Institute

其它主题

Soil microbiology; Bacterial; Isolation & purification; 16s; Ribosomal; Growth & development

语言

英语

类型

Journal Article; Text

自何时收录于AGRIS: 2024-02-27

格式: MODS

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Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots

1999

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书目信息