Analysis of promoters of genes putatively involved in regulation of lupulin and dissecting the role of miRNAs in the regulatory network
2019
Mishra, A. | Siglova, K. | Kocabek, T. | Duraisamy, G. | Patzak, J. | Ono, E. | Matoušek, J.
The regulation of gene expression involves a multifarious regulatory system. Each gene contains a unique combination of cis-acting regulatory sequence elements in the 5' regulatory region that determines its temporal and spatial expression. Recent release of Humulus lupulus (hop), enabled us to predict the possible cis-acting regulatory elements by by scanning 2 kbp of 5' regulatory regions of genes important for lupulin biosynthesis, using Plant CARE, PLACE and Genomatix Matinspector professional databases. The result reveals the probable cis-acting regulatory elements that possibly are involved in the expression genes important for lupulin biosynthesis. In addition, new generation sequencing of small RNAs in hop leads to significant progress in identification of microRNAs that act as predominat regulators of transcription factors (TFs). This study is the first endeavor on mining of miRNAs and analyzing their involvement in secondary metabolism in hop.
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