Single layer centrifugation improves the quality of frozen-thawed sperm of giant panda (Ailuropoda melanoleuca)
2018
Cai, Z.G. | An, J.H. | Liu, Y.L. | Yie, S.M. | Zhang, Y. | Li, F.P. | Chen, J.S. | Wang, X. | Morrell, J.M. | Hou, R.
The reproductive capacity of captive giant pandas is poor and sperm cryopreservation is necessary for the reproduction and conservation of this species. Cryopreservation, however, leads to a significant decrease in sperm quality, including sperm motility, acrosome integrity and DNA integrity. In the present study, a method was developed based on colloid single layer centrifugation that could significantly improve frozen-thawed sperm quality. Two colloids were compared for post-thaw giant panda sperm preparation; the sperm samples had greater total motility (Colloid 1: 44.5 ± 16.0%, Colloid 2: 42.4 ± 10.1% compared with Control: 25.4 ± 8.4%, P < 0.05), linear velocity (Colloid 1: 17.2 ± 8.3 μm/s; Colloid 2: 19.0 ± 9.0 μm/s compared with Control: 6.6 ± 1.7 μm/s, P < 0.05) and membrane integrity (Colloid: 46.9 ± 13.2%; Colloid 2: 54.3 ± 5.7% compared with Control: 36.0 ± 9.1%; P < 0.05). This method could be a useful tool to enable the use of poor quality sperm samples and benefit this population by using available genetic material.
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