Simultaneous Determination of B-Group Vitamins in Enriched Brazilian Dairy Products
2007
Agostini, T.S. | Scherer, R. | Godoy, H.T.
In addition to serving as good natural sources of vitamin B2, milk and milk products are currently being enriched with the vitamins B1, B2, B6, and nicotinamide in Brazil. The objective of this research was to evaluate the levels of enrichment of these products according to their availability in supermarkets in the Campinas (SP, Brazil) region. The separation of the vitamins was carried out on a C 18 column with gradient elution with acetonitrile and aqueous phase. Detection of vitamins was effected in the ultraviolet region and quantification by an external standard. The levels of vitamin B2 determined in powdered whole milk, skimmed milk powder, and one brand of flavored lactic beverage, were practically double those stated on the packages. The same occurred with vitamins B6 and PP (nicotinic acid + nicotinamide), when determined in the same lactic beverage and with vitamin B6, determined in acidified milk. The high levels of vitamin B2, naturally present in whole milk, and the predominance of the free form, allowed for the determination of this vitamin in non-enriched milk.
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