Artemisia scoparia essential oil inhibited root growth involves reactive oxygen species (ROS)-mediated disruption of oxidative metabolism: In vivo ROS detection and alterations in antioxidant enzymes
2012
Kaur, Shalinder | Singh, Harminder Pal | Batish, Daizy Rani | Kohli, Ravinder Kumar
We investigated whether phytotoxicity of Artemisia scoparia essential oil involves a reactive oxygen species (ROS)-mediated stress and alterations in antioxidant enzymes. Effect of Artemisia oil was studied on ROS generation (superoxide anion [O₂ ⁻•], hydrogen peroxide [H₂O₂] content), proline content, root oxidizability and cell death, and ROS metabolism in wheat. Artemisia oil (0.14–0.70 mg/ml) enhanced the levels of H₂O₂, O₂ ⁻•, and proline suggesting induction of oxidative stress. ROS generation was confirmed by in situ detection of cell death, loss of membrane permeability, H₂O₂ accumulation, and lipid peroxidation in intact roots. The activities of the scavenging enzymes- superoxide dismutases, catalases, ascorbate- and guaiacol- peroxidases, and glutathione reductases- were significantly elevated in response to Artemisia oil suggesting their induction as a secondary defense mechanism to scavenge increased ROS generation. The study concluded that Artemisia oil caused root growth inhibition involves ROS-mediated cellular damage despite the activation of antioxidant enzyme levels.
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