Separation and quantitative detection of fermentation γ-polyglutamic acid
2022
Li, Min | He, Yan | Ma, Xia
γ-Polyglutamic acid (γ-PGA) is a kind of polymer material with good biocompatibility and degradability, which is widely used in food, medicine, environmental protection, cosmetics and agriculture. As γ-PGA is produced by microbial fermentation, its separation, purification and quantitative detection are very important. In this study, the separation methods such as organic solvent precipitation, copper salt precipitation and membrane separation precipitation were mainly discussed, and the quantitative determination methods such as spectrophotometer, enzyme method and viscosity method of γ-PGA were discussed and compared. Furthermore, Molecular imprinting as a new method for the separation of γ-PGA has also been analyzed and discussed in order to provide a reference for the separation and purification of γ-PGA.
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