Multilocus isoenzyme diversity among strains of Pseudomonas cepacia isolated from decayed onions, soils, and clinical sources
1994
Yohalem, D.S. | Lorbeer, J.W.
A collection of 59 strains of Pseudomonas cepacia, isolated from a variety of clinical sources, from decayed onions, and from soils were characterized by multilocus isoenzyme electrophoresis and by pathogenic ability on onion. Fifty-three of the strains were found to be electrophoretically unique accessions [D(T)=0.981]. An overall estimate of genetic diversity, H(T), of 0.620 was calculated, which is consistent with other published reports of diversity for the species. Less than two percent of the observed genetic diversity could be attributed to differences between clinical and environmental subpopulations, less than one percent to differences between phytopathogenic and nonphytopathogenic strains. Phytopathogens were absent from the clinical subpopulation. The extreme levels of observed within-group diversity combined with the behavioral differences between clinical and phytopathogenic strains suggest that isoenzymes are sufficient to function as strain identifiers, and that the present species concept of P. cepacia is too broad.
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