Bioactivation of Jhamarkotra rock phosphate by a thermotolerant phosphate-solubilizing bacterium Bacillus sp. BISR-HY63 isolated from phosphate mines
2015
Yadav, Hemendra | Gothwal, Raj Kumar | Mathur, Sujata | Ghosha, Pūrṇendu
Rock phosphate (RP) is a natural source of phosphorus (P), but due to its poor solubility, its application has been restricted to acidic soils. We performed bioactivation of RP in order to increase its efficacy as a fertilizer in direct application to alkaline soils. Simulation of super phosphate (SP) formation was performed, where inorganic acids used in the preparation of SP were replaced by different concentrations of culture supernatant of a thermotolerant phosphate-solubilizing bacterium isolated from RP mines having Fe-P- and Al-P-solubilizing ability to make an environmentally clean SP. Increases in water soluble and citrate soluble P were observed by the treatment of RP with culture supernatant. Organic acid secretion along with decrease in pH was observed and was found to be the major cause in solubilization of RP. HPLC analysis of the culture supernatant showed the presence of citric, malic, and acetic acids. Phylogenetic analysis of the culture showed it to belong to the genus Bacillus .
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