Survival of White Spot Syndrome Virus–Infected Litopenaeus vannamei Fed with Ethanol Extract of Uncaria Tomentosa
2018
Júnior, Osmar Tomazelli | Kuhn, Fernanda | Mendonça Padilha, Paulo José | Mota Vicente, Luiz R. | Winckler da Costa, Sergio | Corrêa da Silva, Bruno | Schleder, Delano Dias | Nunes Nesi, Cristiano | Dal Magro, Jacir | de Lamo‐Castellví, Sílvia
With the present project, the phytochemical composition of Uncaria tomentosa cultivated in Chapecó (Brazil) was analyzed and then the potential of using this local plant extract to protect by oral feeding virus‐infected Litopenaeus vannamei shrimp was also investigated. For this study, five treatments were tested: uninfected shrimp fed with pelleted feed (TC), WSSV‐infected shrimp fed with pelleted feed (T₁), infected shrimp fed with pelleted feed with 1% ethanol extract of U. tomentosa (EEUT) (T₂), infected shrimp fed with pelleted feed with 2% EEUT (T₃), and infected shrimp fed pelleted feed with 4% EEUT (T₄). The chemical analysis of EEUT showed the presence of alkaloids, flavonoids, sterols, terpenes, coumarins, and tannins and also a high in vitro free radical‐scavenging activity as well as high total phenolic content. Shrimp fed with 2 and 4% EEUT (T₃ and T₄) showed a survival probability significantly higher than T₁ and T₂ treatments and no clinical symptoms of WSSV infection. Immunological assay also showed a positive phenoloxidase activity effect on shrimp fed with EEUT.
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