Formation and inhibition of genotoxic glyoxal and malonaldehyde from phospholipids and fish liver oil upon lipid peroxidation
1994
Nishiyama, T. | Hagiwara, Y. | Hagiwara, H. | Shibamoto, T.
Many aldehydes formed from lipid peroxidation are reportedly implicated in various diseases, including arteriosclerosis. Lecithin, a blood plasma lipid, and cod liver oil (known to have a beneficial effect on coronary artery disease) were oxidized by Fenton's reagent. The oxidation products, malonaldehyde (MA) and glyoxal, were analyzed in the presence of alpha-tocopherol or 2"-O-glycosylisovitexin (2"-O-GIV) by gas chromatography after derivatization to 1-methylpyrazol and quinoxaline, respectively. alpha-Tocopherol inhibited MA and glyoxal formation effectively from 5 mg of lecithin at low doses (1 micromole), whereas 2"-O-GIV required 6 micromole to produce the same level of inhibition. However, alpha-tocopherol did not exhibit a satisfactory effect in the case of cod liver oil compared with 2"-O-GIV. Moreover, addition of alpha-tocopherol to cod liver oil increased glyoxal formation slightly. The results of the present study suggested that 2"-O-GIV is an excellent antioxidant for fish oils.
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