Glutathione Transport Systems of the Budding Yeast Saccharomyces cerevisiae
1998
MIYAKE, Tsuyoshi | HAZU, Toshiya | YOSHIDA, Sayaka | KANAYAMA, Munetoshi | TOMOCHIKA, Ken-ichi | SHINODA, Sumio | ONO, Bun-ichiro
The budding yeast Saccharomyces cerevisiae was shown to have two kinetically distinguishable glutathione transport systems. While one with high affinity (GSH-P1; KT=0.045 mM) was regulated, the other with low affinity (GSH-P2; KT>2 mM) was not. GSH-P1 was highly specific to glutathione, and its activity was quickly lost by suspending the cells in buffer solutions. This activity loss was not observed if glucose-containing buffer was used. In addition, ρ ⁻ isolates had only about one half of the glutathione transport activity of the original (ρ ⁺) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. Strong and moderate inhibition of GSH-P1 by protonophores and ionophores, respectively, are attributed to competition for ATP between GSH-P1 and proton- and cation-pumps, respectively.
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