Pheophytin a is a potent suppressor against genotoxin-induced umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002)
1997
Okai, Y. | Higashi-Okai, K.
Previously, a strong antigenotoxic activity was detected in the extract of an edible green alga, Enteromorpha prolifera (Sujiao-nori in Japanese) (Okai et al 1994 Cancer Lett 87, 25-32). The present authors studied the active principle corresponding to the antigenotoxic activity in the extract of this alga, A dark-green spot was detected on a silica gel TLC of the extract of E prolifera which was further purified by a HPLC system and identified to be a derivative of chlorophyll alpha, pheophytin alpha, compared with the retention time of HPLC and absorption spectrum of the standard pheophytin alpha from Anacystis nidulans. Pheophytin alpha derived from E prolifera caused a dose-dependent suppressive effect on umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002) induced by 2-aminoanthracene and 3-amino-1,4-dimethyl-5H-pyrido[4,3-6]indole in the presence of hepatic metabolising enzymes. Furthermore, the standard pheophytin alpha from A nidulans also exhibited a similar inhibitory effect on umu C gene expression system at the same concentrations. These results indicate that pheophytin alpha is a potent suppressor against genotoxin-induced umu C gene expression in S typhimurium (TA 1535/pSK 1002) probably associated with the carcinogenesis.
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