Species-specific detection of Listeria monocytogenes by DNA amplification
1991
Deneer, H.G. | Boychuk, I.
The polymerase chain reaction was used to detect and specifically identify Listeria monocytogenes. A 174-bp region of the listeriolysin O gene was shown to be specifically amplified in L. monocytogenes but not in other species of Listeria or in a number of other gram-positive and gram-negative organisms. Less than 50 organisms could routinely be detected by a procedure involving two rounds of 35 amplification cycles each and without the need for subsequent hybridization with labeled probes.
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书目信息
Applied and environmental microbiology
卷
57
期
2
页码
606
- 609
ISSN
0099-2240
其它主题
Bacterial; Isolation & purification; Base sequence; Bacterial; Molecular sequence data; Disease diagnosis; Heat-shock proteins; Hemolysin proteins; Species specificity
语言
英语
类型
Text; Journal Article
2024-02-27
MODS