Mutation breeding of Emericella foeniculicola TR21 for improved production of tanshinone IIA
2011
Ma, Caixia | Jiang, Dongliang | Wei, Xiying
TR21, an original tanshinone IIA-producing endophytic fungus from the root of Salvia miltiorrhiza Bunge, produces low levels of tanshinone IIA. Three techniques were used to rapidly improve tanshinone IIA production: TR21 mutation by ultraviolet radiation (UV), TR21 mutation by sodium nitrite (NaNO₂) and TR21 mutation by a combination of both UV and NaNO₂. An improved mutant, labeled as NU152, was obtained from the combination of UV and NaNO₂. The content of tanshinone IIA produced by NU152 held a relative high value of 51.44±0.22μg per g, and the NU152 produced a more than 1.46-fold increase in tanshinone IIA yield, compared with the wild type TR21 (P<0.05). The fungus NU152 showed a possible way for the sustainable production of tanshinone IIA.
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