Potentiation of antibody production in Indian major carp Labeo rohita, rohu, by Achyranthes aspera as a herbal feed ingredient
2004
Vasudeva Rao, Y. | Romesh, M. | Singh, A. | Chakrabarti, R.
Labeo rohita, rohu (200+/-17 g), were fed with two types of diets; an experimental diet, containing root extract (0.5%) of Achyranthes aspera (Amaranthaceae) as an ingredient and control diet without the root extract. After 4 weeks of feeding, fish were immunized with chicken RBC. Antigen-specific antibody response, total serum globulin and RNA/DNA ratio of spleen were determined for 4 weeks after immunization. Antigen-specific antibody and total serum globulin levels peaked on day 14 after immunization and gradually decreased towards day 28. Though hemagglutination antibody (HA) titers were always higher in the test group than the control group, total serum globulin level was significantly higher only on days 14 and 21. The sequential relationship between RNA/DNA ratio and protein level was found in the present study, as the RNA/DNA ratio was maximum on day 7 followed by higher serum protein level on day 14 in both groups. The RNA/DNA ratio was significantly (P<0.05) higher in test group of fish than the control group on days 7 and 14. These results showed the immunostimulatory activity of the prepared diet containing root extract of A. aspera.
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