Genotype-dependent responses during in vitro seed germination and establishment of Balanites aegyptiaca (L.) Del. – an endangered agroforestry species
2014
Shahzad, Anwar | Sahai, Aastha
The present study reports variation in in vitro germination responses between two genotypes of Balanites aegyptiaca (L.) Del. an endangered agroforestry species. Fruits were collected from arid and semi-arid zones of Jodhpur (J) and Ahmedabad (A) respectively. Mature seeds of both the germplasms were isolated and subjected to in vitro germination on Murashige and Skoog medium supplemented with gibberellic acid (GA). Marked differences were observed in germination frequency of both genotypes at variable concentrations of GA. Whereas ‘A’ acquired 100% germination frequency on medium containing 1 μM GA, ‘J’ required 2.5 μM GA. Morphogenic variations for seedling traits were also recorded in relation to genotype for shoot, internode and root length along with number of nodes/shoot and leaf size and color. ‘A’ seedlings appeared healthy as compared to ‘J’ seedlings. These morphogenic variations were studied in correlation with the native environmental conditions of both germplasms. In vitro germinated seedlings were hardened and acclimatized in ex situ conditions and survivability was checked during in vitro hardening and ex situ acclimatization in relation to required humidity and shade density respectively.
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