Electrochemical synthesis of reduced graphene sheet–AuPd alloy nanoparticle composites for enzymatic biosensing
2011
Yang, Jiang | Deng, Shengyuan | Lei, Jianping | Ju, Huangxian | Gunasekaran, Sundaram
A simple, fast, green and controllable approach was developed for electrochemical synthesis of a novel nanocomposite of electrochemically reduced graphene oxide (ERGO) and gold–palladium (1:1) bimetallic nanoparticles (AuPdNPs), without the aid of any reducing reagent. The electrochemical reduction efficiently removed oxygen-containing groups in ERGO, which was then modified with homogeneously dispersed AuPdNPs in a good size distribution. ERGO–AuPdNPs nanocomposite showed excellent biocompatibility, enhanced electron transfer kinetics and large electroactive surface area, and were highly sensitive and stable towards oxygen reduction. A biosensor was constructed by immobilizing glucose oxidase as a model enzyme on the nanocomposites for glucose detection through oxygen consumption during the enzymatic reaction. The biosensor had a detection limit of 6.9μM, a linear range up to 3.5mM and a sensitivity of 266.6μAmM⁻¹cm⁻². It exhibited acceptable reproducibility and good accuracy with negligible interferences from common oxidizable interfering species. These characteristics make ERGO–AuPdNPs nanocomposite highly suitable for oxidase-based biosensing.
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