Magnetic Nanospheres Encapsulated by Mesoporous Copper Oxide Shell for Selective Isolation of Hemoglobin
2016
Guo, Zhi-Yong | Zhang, Yue | Zhang, Dan-Dan | Shu, Yang | Chen, Xu-Wei | Wang, Jian-hua
A novel strategy for the preparation of magnetic nanospheres encapsulated by mesoporous copper oxide shell, shortly termed as Fe₃O₄@mCuO, is reported via the calcination of Cu(NH₃)₄(NO₃)₂ into continuous mesoporous CuO shell onto the surface of Fe₃O₄ nanoparticles. The magnetic nanospheres are characterized to possess stable core–shell structure with a crystalline mesoporous CuO layer, exhibiting a CuO loading content of 25.2 ± 1.1% along with a favorable magnetic susceptibility. Fe₃O₄@mCuO nanospheres exhibit favorable selectivity on the adsorption of hemoglobin with a high adsorption capacity of up to 1162.5 mg g–¹. After adsorption, the high magnetic susceptibility allows convenient separation of the nanospheres by an external magnet. The retained hemoglobin could be readily recovered by using 0.5% (m/v) sodium dodecyl sulfate (SDS) as stripping reagent, providing a recovery of 78%. Circular dichroism spectra illustrate virtually no change in the conformation of hemoglobin after the process of adsorption/desorption. Fe₃O₄@mCuO nanospheres are further applied for the selective isolation of hemoglobin from human whole blood, achieving high-purity hemoglobin as demonstrated by SDS-PAGE (polyacrylamide gel electrophoresis) assays.
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