The performance of the microbial fuel cell-coupled constructed wetland system and the influence of the anode bacterial community
2016
Li, Tingting | Fang, Zhou | Yu, Ran | Cao, Xian | Song, Hailiang | Li, Xianning
In order to analyse the influences of substrate and electrode on the performance of microbial fuel cell-coupled constructed wetland (CW-MFC), the electrical generation efficiencies, the decolourization mechanism of reactive brilliant red X-3B, and the microbial communities in the anode were investigated. The closed circuit reactor fed with a mixture of X-3B and glucose (166.7 mg/L X-3B and 140 mg/L glucose) (the mixture CC reactor) got a decolourization rate of 92.79%, which was higher than the open circuit reactor (the mixture OC reactor) and the reactor fed with X-3B (the X-3B reactor). The mixture CC reactor got a maximum power density of 0.200 W/m ³, which was much higher than the X-3B reactor. The intermediates produced by X-3B decolourization were further degraded in CW-MCs. The PCR-denatured gradient gel electrophoresis analysis indicated the dominance of Proteobacteria -like 16S rRNA gnen sequences. The brightest band was detected to be dominant by a Lactobacillus kefiranofaciens -like sequence. The electrogenic bacteria-associated sequences, such as Geobacter metallireducens and Desulfobulbaceae , both existed in the closed circuit and the open circuit reactors, accompanied with Desulfobacterium sp., Klebsiella sp., Aminobacter sp., Flavobacterium sp., Thauera aromatic , and Sphingomonas sp. The abundances of Geobacter sulfurreducens and Betaproteobacteria in the mixture CC reactor were 32.2% and 7.2%, respectively, and were higher than those in the mixture OC reactor. In summary, substrate and electrode can promote the performance of the CW-MFC and have effects on the microbial community in the anode of the CW-MFC.
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