Improvement of Hanseniaspora uvarum biocontrol activity against gray mold by the addition of ammonium molybdate and the possible mechanisms involved
2010
Liu, H.M. | Guo, J.H. | Luo, L. | Liu, P. | Wang, B.Q. | Cheng, Y.J. | Deng, B.X. | Long, C.A.
The efficacy of Hanseniaspora uvarum against gray mold by adding ammonium molybdate (NH₄-Mo) and the mode of actions were evaluated. The results showed that H. uvarum at 1×10⁶ CFUml⁻¹ plus 1mmoll⁻¹ NH₄-Mo greatly reduced gray mold in grape fruits. NH₄-Mo at concentrations of 1, 5, 10 and 15mmoll⁻¹ significantly inhibited spore germination and mycelium growth of Botrytis cinerea. Population growth of H. uvarum was markedly inhibited by NH₄-Mo at 5mmoll⁻¹ in vitro and not affected by addition of NH₄-Mo at 1 and 5mmoll⁻¹ in wounds combination of NH₄-Mo and H. uvarum induced higher activities of peroxidase (POD), polyphenoloxidase (PPO), phenylalanine ammonialyase (PAL), superoxide dismutase (SOD), catalase (CAT) and β-1,3-Glucanase than individual application of H. uvarum or NH₄-Mo. The enhancement of disease control may be directly because of the inhibitory effects of NH₄-Mo on spore germination and mycelial growth of B. cinerea in vitro, and indirectly because of the induced defense reactions by NH₄-Mo in grape berries.
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