Overexpression of NADH oxidase gene from Deinococcus geothermalis in Escherichia coli
2013
Kazuya, Sase | Tomomi, Iwasaki | Hatsune, Karasaki | Masahide, Ishikawa
When using stable enzyme genes from a thermophile to create a biosensor in Escherichia coli, it is vital that these genes be overexpressed in order to provide a sufficient supply of enzymes. In this study, overexpression of the NADH oxidase (Nox) gene from the thermophile Deinococcus geothermalis was successfully achieved with the aim of creating a stable biosensor active at room temperatures. To do so, modification of 10 nucleotides, GAAATTAACT, upstream of the start codon of the Nox gene was necessary.
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书目信息
Journal of Environmental Sciences
卷
25
ISSN
1001-0742
出版者
Routledge
其它主题
Gene overexpression; Deinococcus geothermalis; Start codon; Overexpression; Enzyme stability; Ambient temperature; Nad (coenzyme); Stable enzyme
语言
英语
注释
2019-12-06
类型
Journal Article; Text
2024-02-27
MODS