The role of spray-drying atmosphere on fridericia chica (bonpl.) L.G. Lohmann standardized extract production for wound healing activity
2021
Sousa, Ilza Maria de Oliveira | Rivera, Gerardo Alvarez | Pinto, Diana | Queiroz, Nubia de Cássia Almeida | Bastos, Veronica Isabel Correia | Braga, Lucia Elaine O. | Palmeira, Josman Dantas | Amaral, Helena | Ferreira, Helena Maria Neto | Oliveira, Helena Cristina Correia | Gonçalves, Fernando J Mendes | Ruiz, Ana Lucia TG | Ibáñez, Elena | Oliveira, Beatriz PP | Foglio, Mary A.
Fridericia chica (Bonpl.) L.G. Lohmann (synonym Arrabidaea chica Verlot) is widely used in Brazilian folk medicine. Considering overcoming pitfalls of scaling up production of plant extracts, herein the effects of N₂ atmosphere for extract spray-drying process is reported. Samples were monitored by in vitro antioxidant activity and microbiological evaluation. The drying atmosphere influenced 3-deoxyanthocyanines content when using air as atomizing gas, decreasing carajurin (37.5%) content with concomitant increase in luteolin yield (24.1%). Both drying processes preserved the pharmacological activity. In the cell migration test with HaCaT cells, the extract dried under air flow (5 μg/mL) promoted wound closure by 78% (12 hours) whereas the extract dried using N₂ flow promoted 49% (12 hours), with 98% closure (12 hours) for the positive control. The antimicrobial evaluation for Staphylococcus aureus did not differ within drying atmospheres, with MIC (minimum inhibitory concentration) at 0.39 mg/mL. Therefore, the drying process reported herein did not interfere with the biological activity’s outcome.
显示更多 [+] 显示较少 [-]