Effects of stem injections of gibberellin A4/7 and paclobutrazol on sex expression and the within-crown distribution of seed and pollen cones in black spruce (Picea mariana)
1998
Smith, R.F.
Two experiments in black spruce (Picea mariana (Mill.) BSP) seedling seed orchards were established to determine if a stem injection of paclobutrazol (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2, 4-triazol-1-yl) could be used as an adjunct treatment to increase the efficacy of stem injections of gibberellins A4 and A7 (GA4/7). Trees received a single injection of GA4/7 and (or) paclobutrazol shortly after vegetative bud burst. There was a dose-dependent but nonlinear increase in the production of cones of both sexes in response to stem injections of either GA4/7 or paclobutrazol. The optimum rate of GA4/7 for stimulating pollen-cone production was 3.3 mg, whereas the most seed cones were induced on trees receiving 11 mg. The sex ratio (number of seed cones/number of pollen cones) increased with the rate of GA4/7 applied. Injecting paclobutrazol also promoted cones of both sexes equally, resulting in sex ratios comparable with that of the control trees. Treatments did not affect the total numbers of buds (vegetative, latent, and cone) produced. Seed- and pollen-cone buds occurred in positions that would have otherwise developed vegetatively and become latent, respectively. The mechanisms whereby paclobutrazol could affect flowering in black spruce are discussed. The use of paclobutrazol as an adjunct to GA4/7 treatments in black spruce seedling seed orchards appears effective, practical, and safe.
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