Functional responses of aquatic invertebrates to anthropogenic stressors in riparian zones of Neotropical savanna streams

Riparian zones ensure freshwater ecosystem processes such as microclimate regulation, organic matter inputs, and fine substrate retention. These processes illustrate the importance of riparian zones for freshwater ecosystem functioning, maintaining biodiversity, and mitigating the effects of anthropogenic pressures on aquatic ecosystems. We aimed to determine the freshwater invertebrate biological traits that are most affected by anthropogenic stressors in the riparian zones of 210 Neotropical savanna headwater streams. We assessed % canopy cover over the streambed, % fine bottom substrate, % leaf pack, substrate heterogeneity, and water temperature. Firstly, we identified bioindicator taxa in response to each local metric gradient. We assessed the functional response, based on biological traits of bioindicators previously selected. We identified 324,015 specimens belonging to 84 freshwater invertebrate taxa. Fifty-one taxa (60%) were bioindicators of anthropogenic stressors. We found three main sets of traits. (1) a set of traits linked to increased disturbance (higher percentage of fine sediments), consisting of organisms with aquatic adult stages, spherical body shape, and long adult life stages. (2) A set of traits linked to lower disturbance (higher substrate heterogeneity), including taxa with short or very short lifespans that live attached to substrates. (3) A set of traits linked to higher water temperature, including organisms with short adult lifespans and lower body flexibility. These patterns suggest that the stressors act as environmental filters and do not act independently on single traits, but rather, selecting sets of biological traits that facilitate taxa surviving and persisting in local environmental conditions. Our results support the development of powerful evaluation tools for environmental managers and decision makers. Because degraded freshwater communities respond in similar ways across large biogeographic areas, these sets of traits can be used for ecological monitoring efforts along other tropical savanna headwaters worldwide.