Peroxidases during adventitious rooting in cuttings of Arbutus unedo and Taxus baccata as affected by plant genotype and growth regulator treatment
2004
Metaxas, D.J. | Syros, T.D. | Yupsanis, T. | Economou, A.S.
Cuttings of Arbutus unedo (strawberry tree) and Taxus baccata (yew) were treated with 8.0 and 10.0 g l-1, respectively, of KIBA, IBA, IAA, NAA and Paclobutrazol. No rooting occurred without growth regulator treatment. The effect of growth regulators on percentage of rooting followed the order KIBA > IBA > IAA = NAA = Paclobutrazol = 0% (for A. unedo) and KIBA > IBA > IAA > NAA > Paclobutrazol = 0% (for T. baccata). Genotypes of the above plant species had significant effects on the number and length of roots, percentage of rooting and peroxidase specific activity (PA) on KIBA-treated cuttings. High PA seems to be related with low percentage of rooting in the case of A. unedo cuttings while no similar results were noticed in the case of T. baccata. Electrophoretic analysis revealed the appearance of two to three anionic and one cationic peroxidase isoforms in A. unedo cuttings, while six to nine anionic and no cationic peroxidases isoforms appeared in the case of T. baccata genotypes. During adventitious rooting, the PA showed the three interdependent phases (induction, initiation, expression) in both K-IBA treated cuttings of A. unedo and T. baccata, but in a different time course.
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