A new method for monitoring the susceptibility of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae), to pymetrozine by combining topical application and measurement of offspring number
2016
Tsujimoto, Katsuhiko | Sugii, Shinji | Sanada-Morimura, Sachiyo | Matsumura, Masaya
Pymetrozine is a promising chemical agent for controlling the brown planthopper, Nilaparvata lugens (Stål), after resistance to some neonicotinoid insecticides developed nearly a decade ago. Susceptibility tests by assessing mortality resulting from insecticidal activity are not available for pymetrozine because its key biological action suppresses the reproductive performance of treated N. lugens females. In this study, a new simple method for monitoring the susceptibility of N. lugens to pymetrozine was developed by combining topical application and assessment of the number of offspring using young rice seedlings in a test tube. The ED₅₀ values of the Osaka strain (collected in 1971) and Kumamoto strain (collected in 2011) of N. lugens against pymetrozine were 0.036 and 0.091 μg/g, respectively. In the future, this method will be used to monitor the susceptibility of N. lugens to pymetrozine.
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