Enrichment of gamma-linolenic acid from fungal oil by lipase-catalysed reactions
1991
Mukherjee, K.D. | Kiewitt, I.
Various lipases have been evaluated as biocatalysts for the enrichment of gamma-linolenic acid from a commercial fungal oil derived from Mucor sp. by selective esterification of the fungal oil fatty acids with n-butanol or by selective hydrolysis of the oil. Lipase from M. miehei (Lipozyme), as compared to lipases from Candida cylinelracea, Penicillium cyclopium, and Rhizopus arrhizus, was found to be most effective in the enrichment of gamma-linolenic acid in unesterified fatty acids upon esterification of the fungal oil fatty acids with n-butanol. Thus, the gamma-linolenic acid content could be raised from 10.4% in the starting material to 68.8% in the unesterified fatty acids. Selective hydrolysis of the fungal oil triacylglycerols using Lipozyme resulted in about 1.5-fold enrichment of gamma-linolenic acid in the unhydrolysed acylglycerols. Other lipases tested, such as those from P. cyclopium, C. cylindracea, R. arrhizus, Penicillium sp. (Lipase G), porcine pancreas and Chromobacterium viscosum, were also rather ineffective in the enrichment of gamma-linolenic acid by selective hydrolysis of the fungal oil triacylglycerols.
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