Cytotoxicity of benalaxyl, metalaxyl, and triadimefon on Chinese hamster ovary cells
2014
Al-Sarar, Ali S. | Bayoumi, Alaa E. | Abobakr, Yasser | Hussein, Hamdy I.
The cytotoxicity of the fungicides benalaxyl, metalaxyl, and triadimefon was evaluated in vitro using the Chinese hamster ovary (CHO-K1) cell line. The midpoint cytotoxicity values of neutral red (NR) incorporation (NRI ₅₀), total cellular protein content (TCP ₅₀), and the methyl tetrazolium assay (MTT ₅₀) were estimated. Benalaxyl was the most cytotoxic fungicide, followed by metalaxyl and triadimefon. Fetal calf serum (10%) caused a reduction in benalaxyl, metalaxyl, and triadimefon cytotoxicity by factors of 1.8, 1.3, and 1.3. The effects of sublethal concentrations (NRI ₂₅) of the three fungicides on the glutathione redox cycle components glutathione S -transferase, glutathione reductase, glutathione peroxidase, and total glutathione content were studied. The ameliorative effects of extracellular glutathione (1 mmol L ⁻¹), vitamin C (70 µ mol L ⁻¹), and vitamin E (30 µ mol L ⁻¹) were also investigated. The three antioxidants led to significant effects on the glutathione redox cycle components.
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