Changes in the apolipophorin-III level in the hemolymph of Galleria mellonella larvae after bacterial infection
2011
Andrejko, Mariola | Mizerska-Dudka, Magdalena
The level of apoLp-III in hemolymph of Galleria mellonella larvae infected with entomopathogenic strains of Pseudomonas aeruginosa, Serratia marcescens, Bacillus thuringiensis var. kurstaki, Bacillus thuringiensis var. alesti or non-pathogenic bacterium Escherichia coli were studied. It was found that the amount of 18 kDa protein decreased within infection with P. aeruginosa and S. marcescens in comparison to its content in the hemolymph of non-infected larvae. Whereas, the inconsiderable increase of the apoLp-III level was observed in the hemolymph of infected larvae as a response to injection with B. thuringiensis. There was no difference in the apoLp-III level between hemolymph collected from larvae infected with E. coli and control naïve insects. In vitro studies also demonstrated apoLp-III degradation by P. aeruginosa and S. marcescens culture supernatants.
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