Gynogenic response of muskmelon genotypes with temperature and MS medium supplementations
2019
Kaur, H. A. | Sharma, S. P. | Kaura, Ainna. | Kalia, A.
Gynogenesis, megaspore culture technique can generate haploid plants from ovules, (the female gametes) and it requires less time besides ensuring higher potential for generation of haploid plants. This study compares the gynogenic response of two genotypes of Cucumis melo var. reticulatus, ‘MS-5’ and ‘MM Sel. 103’ on three modified MS media, TDZ (at 0.02, 0.04 mg L(‑1)), and coconut water + proline + 2,4-D+kinetin. Ovaries of both the genotypes were subjected to two temperature treatment steps i.e., heat treatment at 35°C for 2 days and cold treatment at 4°C for 4 and 8 days. Both genotypes varied in their response to temperature and MS medium supplementations. Genotype ‘MM Sel. 103’ exhibited highest callusing rate on coconut water + proline + 2,4-D+kinetin supplementation without any temperature treatment. However, heat treated explants showed best results on media supplemented with TDZ at 0.04 mg L(‑1). While, ‘MS-5’ genotype, showed better callusing rate on TDZ supplemented MS media (at 0.02 mg L(‑1)) on heat and cold treatment (for 4 days). The generated calli differentiated and mimicked different stages of embryos as observed under stereomicroscope. Embryo count done post 15 days of culturing exhibited higher number of embryo and ovule like structures in ‘MM Sel. 103’ genotype as compared with ‘MS-5’. However, the size of ovules or embryos was the same in both the genotypes. This work accentuates the need for optimization of genotype specific modulations in culturing media and pre-treatment protocols for successful generation of haploid through gynogenesis in muskmelon.
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