First Application of a Microsphere-Based Immunoassay to the Detection of Genetically Modified Organisms (GMOs): Quantification of Cry1Ab Protein in Genetically Modified Maize
2007
Fantozzi, A. | Ermolli, M. | Marini, M. | Scotti, D. | Balla, B. | Querci, M. | Langrell, S.R.H. | Eede, G van den
An innovative covalent microsphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 genetically modified (GM) maize lines. In particular, a specific protocol was developed to assess the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% [weight of genetically modified organism (GMO)/weight[. Test linearity was achieved in the range of values from 0.1 to 3%, whereas fluorescence signal increased following a nonlinear model, reaching a plateau at 25%. The limits of detection and quantification were equal to 0.018 and 0.054%, respectively. The present study describes the first application of quantitative high-throughput immunoassays in GMO analysis.
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