Characterization of a Vivipary 1 homologue in wheat
1999
Sorrells, M.E. | Benscher, D. | Wilson, W.A. | Zhu, J. | Graznak, S. | Lupold, D.S.
The historical association of white kernel color in wheat with susceptibility to preharvest sprouting and the comparative mapping information suggesting the possible involvement of maize (Zea mays L.) vivipary 1 were used as the basis for isolation and characterization of the wheat homologue. Consensus primers were designed based on conserved regions of the gene in rice (Oryza sativa L.), maize, and Arabidopsis. These primers were used to amplify a portion of the wheat vivipary 1 gene for sequencing and analysis. A comparison of sequence similarity between maize, rice, wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) revealed that exons 1 and 6 are highly diverged among all the species. Sequence divergence for rice/maize/barley comparisons are similar across all exons. Comparisons with wheat indicate a trend for exons 2 and 5 to be slightly less conserved leading to lower sequence similarity overall for wheat. Barley sequences were all closely related to wheat, even for exon 6. If additional mapping data support a recent report that Vp1 is not responsible for red kernel color in wheat, this could facilitate enhancement of grain dormancy in white wheat by allowing manipulation of vivipary 1 without affecting kernel color.
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