Genetic variability of BoLA-DRB3.2 gene in Kenkatha breed of cattle assessed by PCR-SSCP
2013
Ranjan, Rakesh | Bhong, C.D. | Chavan, K.N. | Parmar, S.N.S. | Joshi, C.G.
Present study was conducted to investigate BoLA (Bovine leucocyte antigen) gene polymorphism in indigenous draft Kenkatha (n=52) breed of cattle at DRB3.2 loci by PCR-SSCP (Single strand conformational polymorphism). This BoLA region encodes cell surface glycoprotein, which binds to foreign antigens of T lymphocytes that play central role in the induction and regulation of adaptive immunity. A PCR-SSCP of 284bp amplicon of BoLA-DRB3.2 gene revealed 13 SSCP patterns. This suggests that exon 2 region of BoLA-DRB3 is highly polymorphic in nature. This may be due to natural selection on a BoLA allele pool, leading to the development of resistance to local infectious diseases.
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