Simultaneous determination of urea and ammonia nitrogen in soil extracts and water by high performance liquid chromatography
1988
Abshahi, A. | Goyal, S.S. | Mikkelsen, D.S.
Combining the separation efficiency of high performance liquid chromatography (HPLC) and the catalytic solid phase urease reactor, a simple, sensitive, and highly specific method for simultaneous determination of urea and ammonia nitrogen is possible. In this system the immobilized urease catalyzes the hydrolysis of urea to ammonium ions, which is then measured fluorometrically after postcolumn derivatization with o-phthalaldehyde (OPA). The fluorophore is detected on-line with a fluorometric detector, 344(ex) and 455(em) nm. The method is rapid and can be used to accurately monitor the urea and ammonium-N content of soil, water, and fertilizer materials.
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