Compact multi-enzyme pathways in P. pastoris

Geier, Martina; Fauland, Pia; Vogl, Thomas; Glieder, Anton

Compact multi-enzyme pathways in P. pastoris

2015

Geier, Martina | Fauland, Pia | Vogl, Thomas | Glieder, Anton

We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.

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