HPLC-MS Analysis of Isoniazid in Dog Plasma
2007
Wang, Anna | Zhang, Wei | Sun, Jin | Liu, Jianfang | Sang, Yanshuang | Gao, Shuo | He, Zhonggui
A simple, rapid, and sensitive liquid chromatography-mass spectrometric (LC-MS) method was developed and validated for the determination of isoniazid in dog plasma. Plasma samples were deproteined with methanol and separated on a C₁₈ column interfaced with a single quadrupole mass spectrometer, using 0.1% formic acid-acetonitrile (91:9 v/v) as mobile phase. Detection was performed by positive electrospray ionization with selected ion monitoring at m/z 138 for isoniazid and 152 for entecavir maleate internal standard. Linearity was obtained over the range of 25-5,000 ng mL-¹, with a lower limit of quantification of 25 ng mL-¹. The intra- and inter-day precision was less than 2.7% in terms of relative standard deviation. Accuracy, expressed as relative error, ranged from -2.0 to 8.0%. Plasma samples were analysed within 5 min. The method was successfully applied to the evaluation of the pharmacokinetics of isoniazid in dog plasma.
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