A protocol for rapid DNA extraction fromArabidopsis thaliana for PCR analysis
2004
Kasajima, Ichiro | Ide, Yoko | Ohkama-Ohtsu, Naoko | Hayashi, Hiroaki | Yoneyama, Tadakatsu | Fujiwara, Tōru
We present a method for instant DNA extraction fromArabidopsis thaliana based on a simple DNA extraction method (Edwards et al., 1991). A piece of rosette leaf (typically 3–5 mg) was ground in a centrifuge tube in extraction solution. Extracted DNA was suitable for PCR analysis, without centrifugation. The feasibility of this method was confirmed by testing 24 primer sets. This method requires less than 1 mg of plant tissue and is useful for genetic mapping, transgene detection, and other experiments.
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