Studies on the prevalence, clinico-pathology and molecular detection of chicken infectious anaemia (CIA) virus in poultry in Mizoram, India
2018
Deka, Abhijit | Rajkhowa, T.K. | Singh, Y.D. | Ravindran, R. | Arya, R.S.
Chicken infectious anaemia is an economically important disease of chicken characterized by stunted growth, anaemia and lymphoid depletion that result in immuno-suppression in affected birds. Although this disease has been reported from other parts of India, nonetheless, information from North Eastern Region (NER) of India is lacking. Thus, the present study was aimed to study prevalence, clinico-pathology and molecular detection of chicken infectious anaemia (CIA) virus in the poultry population of Mizoram. A total of 3635 samples from 16 different farms with history of poor growth followed by increasing rate of mortality were studied. Results have shown that 10 out of 16 firms who were under investigation had CIAV outbreaks which were confirmed by PCR. Clinically the disease appeared as haemorrhagic dermatitis, generalized anaemia, stunted growth, curled toe paralysis. Gross lesions were characterized by subcutaneous haemorrhages in the thigh muscles, around atlanto-occipital joint, pale and anaemic viscera, atrophied thymus, pale, watery and fatty bone marrow,. Histopathological examination of tissues revealed generalized lymphocytic depletion in the thymus, spleen and bursa of Fabricius. Eosinophilic intranuclearinclusion bodies were observed in thymus, bursa of Fabricius and bone marrow. Diagnosis of the disease was confirmed by detection of 713 bp region of the VP2 gene of chicken infectious anaemia virus in tissue lesions using PCR assay.
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